The seven lakes of San Pablo City, Laguna are rich in aquatic insect diversity. Around 46 species of insects (26 families and 7 orders) were identified with Cleon sp. (O. Ephemeroptera, F. Baetidae) as the most abundant. Pollution tolerant species dominated Lakes Calibato, Bunot, Mohicap and Sampaloc. Among the environmental variables, total dissolved solids, conductivity, and dissolved oxygen were the top factors associated with community variation across the lakes. Dissolved oxygen and salinity were the most important predictors for species richness and abundance.